Transgenic Mice Expressing Maturation and Selenoprotein Synthesis in Selective Inhibition of Selenocysteine tRNA
نویسندگان
چکیده
10.1128/MCB.21.11.3840-3852.2001. 2001, 21(11):3840. DOI: Mol. Cell. Biol. Gladyshev and Dolph L. Hatfield Berry, Alan M. Diamond, Byeong Jae Lee, Vadim N. Feigenbaum, David B. Mansur, Raymond F. Burk, Marla J. Harney, Kristina E. Hill, Gerald F. Combs, Lionel El-Saadani, Gregory V. Kryukov, Qi-An Sun, John W. Mohamed E. Moustafa, Bradley A. Carlson, Muhammad A. Selenocysteine tRNA Isopentenyladenosine-Deficient Transgenic Mice Expressing Maturation and Selenoprotein Synthesis in Selective Inhibition of Selenocysteine tRNA
منابع مشابه
Selective inhibition of selenocysteine tRNA maturation and selenoprotein synthesis in transgenic mice expressing isopentenyladenosine-deficient selenocysteine tRNA.
Selenocysteine (Sec) tRNA (tRNA([Ser]Sec)) serves as both the site of Sec biosynthesis and the adapter molecule for donation of this amino acid to protein. The consequences on selenoprotein biosynthesis of overexpressing either the wild type or a mutant tRNA([Ser]Sec) lacking the modified base, isopentenyladenosine, in its anticodon loop were examined by introducing multiple copies of the corre...
متن کاملThe selenocysteine tRNA STAF-binding region is essential for adequate selenocysteine tRNA status, selenoprotein expression and early age survival of mice.
STAF [Sec (selenocysteine) tRNA gene transcription activating factor] is a transcription activating factor for a number of RNA Pol III- and RNA Pol II-dependent genes including the Trsp [Sec tRNA gene], which in turn controls the expression of all selenoproteins. Here, the role of STAF in regulating expression of Sec tRNA and selenoproteins was examined. We generated transgenic mice expressing ...
متن کاملSpecific excision of the selenocysteine tRNA[Ser]Sec (Trsp) gene in mouse liver demonstrates an essential role of selenoproteins in liver function.
Selenium is essential in mammalian embryonic development. However, in adults, selenoprotein levels in several organs including liver can be substantially reduced by selenium deficiency without any apparent change in phenotype. To address the role of selenoproteins in liver function, mice homozygous for a floxed allele encoding the selenocysteine (Sec) tRNA([Ser]Sec) gene were crossed with trans...
متن کاملSelective restoration of the selenoprotein population in a mouse hepatocyte selenoproteinless background with different mutant selenocysteine tRNAs lacking Um34.
Novel mouse models were developed in which the hepatic selenoprotein population was targeted for removal by disrupting the selenocysteine (Sec) tRNA([Ser]Sec) gene (trsp), and selenoprotein expression was then restored by introducing wild type or mutant trsp transgenes. The selenoprotein population was partially replaced in liver with mutant transgenes encoding mutations at either position 34 (...
متن کاملLoss of housekeeping selenoprotein expression in mouse liver modulates lipoprotein metabolism.
Selenium is incorporated into proteins as selenocysteine (Sec), which is dependent on its specific tRNA, designated tRNA([Ser]Sec). Targeted removal of the tRNA([Ser]Sec) gene (Trsp) in mouse hepatocytes previously demonstrated the importance of selenoproteins in liver function. Herein, analysis of plasma proteins in this Trsp knockout mouse revealed increases in apolipoprotein E (ApoE) that wa...
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